Biotechnology Fybsc 254q42
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Academic Council 25/05/2011 Item No. 4.51
UNIVERSITY OF MUMBAI
Syllabus for the F.Y.B.Sc. Program: B.Sc. Course : Biotechnology (Credit Based Semester and Grading System with effect from the academic year 2011–2012)
SEMESTER I (Theory) USBT101 PAPER I Unit – I: Microbiology Topic Subtopic A) Nutrition, Nutritional requirements, isolation Nutritional classification of microorganisms. cultivation and Design of culture media, types of culture media. preservation of Concept of isolation, methods of isolation- natural microorganisms microbial populations (mixed culture). Cultivation of microorganisms , Methods of enumeration, Preservation of microorganisms (Principles and method) B) Sterilization i) Principle and application – methods of and disinfection sterilization (physical, chemical, filtration, heat, radiation, gaseous, etc). ii) Disinfection – ideal disinfectant, list of disinfectant, and their application – evaluation of disinfection (Phenol coefficient) Unit – II: Genetics Topic Subtopic A) Mendelian i)Segregation- Mendels experiments, terminology, principles testing phenotypes, examples of gene differences and segregation. ii) Independent assortment – genotypes of dihybrid crosses, testing dihybrid genotypes, crosses involving 3 -4 gene differences, correspondence between mendelian factors and chromosome, symbols, segregation and assortment in haploid organisms. B) Mutations Types of mutations, mutagen, types of mutagens, and mutagenic molecular basis of mutagenesis, reversion, agents induced and spontaneous mutation and silent mutation. Unit – III: Microbial diversity and Functional Biology Topic Subtopic A) Different i) Archaebacteria groups of ii) Eubacteria prokaryotes iii) Blue green algae, Actinomycetes, Eumycota B) Plants Plant cell, types of plant tissues and their importance, organization on angiosperms and modifications of organs of commercial importance Storage roots like sweet potato, carrot, beetroot, raddish. Storage stems – potato, ginger, turmeric, yams, onion, garlic, Acorus, Fruits – Parthenocarpy Seeds – (lectin) red bean
No. of Lectures 45 15
15
15
No. of credit 2
SEMESTER I (Theory) USBT102
Topic A) Ultra structure of prokaryotes B) Ultra structure of Eukaryotic cell Topic A) Chemical bonds and Role of water
PAPER – II Unit – I: Cell Biology Subtopic Flagella, Pilli, capsule, cell wall, cell membrane, outer membrane, cytoplasm, endospores, reserve material, mesosome, nuclear material, plasmid extrachromosomal material Structure of Fungal cell and Yeast – Cell wall, plasma membrane.
Unit – II: Biomolecules Subtopic Covalent bonds, dipoles, ionic, hydrogen bonds. Hydrophobic interaction, Vander Waals forces, functional group. Structure and properties of water, pH – pH meter, Buffers.
No. of Lectures 45 15
15
B) D & L Glyceraldehydes, structure of Carbohydrates monosaccharide, disaccharides, and polysaccharides. Isomers of monosaccharides, chemical/physical properties of carbohydrate, chemical reactions for detection of mono., di and polysaccharides C) Nucleic acids
Structure, function of NA, properties and types of DNA, RNA, structure of polynucleotides
Unit – III: Medical Biotechnology Subtopic A) What is biotechnology? Introduction Biotechnology as an interdisciplinary pursuit. to Scope of biotech in various areas. Biotechnolog Public perception of biotechnology. y and its areas Biotechnology and the developing world. of application B) HostHost parasite relations, infections: sources, Microbe methods of transmission, virulence factors, basic interaction types of clinical infections, Topic
15
No. of credit 2
SEMESTER –I (Practicals) USBTP1
No. of credits
No. 1
2.
3. 4. 5.
6.
PAPER I Title Study of Microscope, dark field microscope and all Lab equipmentsautoclave, hot air oven, centrifuge, incubator, rotary shaker, filter assembly, LAF, pH meter Media preparation and sterilization –Nutrient broth/agar, Sabourauds broth/agar, MacConkeys broth/agar, Superimposed imposed blood agarslant and plate Aseptic transfer of sterile Nutrient Agar/St. Nutrient broth Study of plant tissues from suitable material- parenchyma, sclerenchyma, xylem and phloem. Enrichment (Allen and Chu) of algae and permanent slides of - Nostoc, Anabaena, Spirullina, Chorella
1
Slide culture technique for Actinomycetes
SEMESTER –I (Practicals) USBTP1
No. of credits
3
PAPER II Title Simple staining – Monochrome stain with basic fuchin, crystal violet, malachite green, safranin.-yeast. Differential staining – Grams staining of mixture (gram positive and gram negative) Study of fungi (Aspergillus niger) and yeast.
4 5
Special staining – capsule, cell wall, lipid, spores, nucleus Motility –hanging drop, stab culture.
6
Qualitative tests for biomolecules – carbohydrates, lipids
7 8
Isolation of organism from stool/feces on selective medium (Mac Conkey agar) Gram staining of organism from saliva and skin.
9
Extraction of Pectin from any suitable fruit.
10
Staining of Starch grain from potato
No. 1 2
1
Total number of credits
2.0
SEMISTER II (Theory) USBT201
Topic A) The Microscope
B)Stains and Staining solution
PAPER – I Unit – I: Microbiology Subtopic Lenses and bending of light, Simple and compound microscope, Light Microscope (Bright –field microscope) Parts of light microscope- Eye piece, objectives, condensors, numerical aperture, resolving power, Dark field microscope.
No. of No. of Lectures credit 45 2 15
Definition of dye, chromogen. Structure of dye. Chromophore and auxochrome groups. Preparation and staining of specimens, Fixation, Dyes and simple stains, Leuco compound, Monochrome staining, Mordant, Differential staining (Gram’s and Acid-fast), Romanowsky’s stain, natural dyes. Unit II:Genetics Subtopic Transformation- Griffith’s exp. Conjugation-Davis experiment, Transduction-generalised (basic concept)
15
Unit– III: Microbial diversity and Functional Biology Topic Subtopic A) Histology Animal cell, tissues – epithelial tissues, connective of tissues, muscular tissues, nervous tissues. mammalian tissues B) What organisms are suitable for genetic Experimental experimentation: eukaryotes and prokaryotes. models Criteria for selection Maintenance of Drosophila, Albino mice, Guinea pigs, Hamsters, Monkey, Saccharomyces cerevisiae, neurospora crassa, Zea mays, Pisum sativum, E.coli
15
Topic A) Gene transfer mechanisms in bacteria B) Extension Multiple alleles-blood group, modification of of genetic dominant relationships, gene interactions, essential analysis and lethal genes, gene expression and environment(temperature, light, hormones)
SEMESTER II (Theory) USBT202
Topic A) Cytoplasmi c organelles B) Interface nucleus including (Cell cycle)
Topic A) Lipids
B) Amino acids, proteins and c0 C) Enzymes
Topic A) Immunity
B) Antigens and antibody
PAPER II Unit– I: Cell Biology Subtopic Introduction to cytoplasmic Mitochondria and chloroplast.
No. of Lectures 45 15 organelles-
Nucleosome, biological significance of DNA, cell cycle- phases, regulation by cell growth and extra cellular signals, cell cycle check points, coupling of s phase to m phase, cell cycle progression. Unit – II: Biomolecules Subtopic Classification and properties, saturated, unsaturated, structure and function triacylglycerol, storage lipids, structural lipids, phospholipids, action of phospholipases, steroids.
15
Structure, properties, function, and chemical reaction of amino acids, classification and structure of proteins. Silk fibroin, keratin hemoglobin and myoglobin. Structure of peptides. Titration curve of amino acids. Concept of Isoelectric pH, zwitter ion. Introduction, classification, active site and enzyme specificity Unit – III: Medical Biotechnology Subtopic Introduction, mechanism of innate immunity, acquired immunity, local and herd immunity. Humoral and cellular immunity. Determinant of antigenicity, biological classes Structure, immunoglobulin classes.
15
No. of credit 2
SEMESTER II (Practicals) USBTP2
No. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
PAPER I Title Study of minimum nutritional requirement. Isolation techniques of E.coli/ S. aureus Preservation of microorganisms – serial subculture method and paraffin oil technique. Serial dilution technique – i) Surface spread ii) pour plate. Enumeration methods – haemocytometer, breeds count, opacity tube Effects of environment on bacterial growth – effect of pH, temp, osmotic pressure Study of aerobic microflora and anaerobic microflora from (cow dung) in Robertson’s cooked meat medium Drosophila culture-corn meal medium Mounting of Squamous and stratified epithelium Study of animal tissues from permanent slides:-blood, bone marrow, neuron, connective tissue-spongy, smooth, skeletal and heart muscles. SEMESTER II (Practicals) USBTP2
No. 1 2 3 4 5
No. of credit 1
PAPER II Title Qualitative tests for biomolecules – protein, nucleic acids, amino acid Amylase, urease, invertase, catalase, dihydrogenase activity (qualitative) Extraction of Casein from milk. Stages of Mitosis Meat tenderization using papain. Total number of credits
No. of credit 1
2.0
Distribution of Topics and Credits F.Y.B.Sc. Biotechnology Semester I Course USBT1O1
Nomenclature Microbiology, Genetics and Microbial Diversity and Functional Biology
USBT1O2
Cell Biology, Biomolecules and Medical Biotechnology
USBTP1
Microbiology, Genetics and Microbial Diversity and Functional Biology Cell Biology, Biomolecules and Medical Biotechnology Practical I & II
Credits 02
Topics 1. Microbiology 2. Genetics 3. Microbial Diversity and Functional Biology
02
1. Cell Biology 2. Biomolecules 3. Medical Biotechnology
02
F.Y.B.Sc. Biotechnology Semester II Course USBT2O1
USBT2O2
USBTP2
Nomenclature Microbiology, Genetics and Microbial Diversity and Functional Biology
Cell Biology, Biomolecules and Medical Biotechnology Microbiology, Genetics and Microbial Diversity and Functional Biology Cell Biology, Biomolecules and Medical Biotechnology Practical I & II
Credits
02
02
02
Topics 1. Microbiology 2. Genetics 3. Microbial Diversity and Functional Biology 1. Cell Biology 2. Biomolecules 3. Medical Biotechnology
Scheme of Examination: The performance of the learners shall be evaluated into two parts. The learner’s performance shall be assessed by Internal Assessment with 40% marks in the first part & by conducting the Semester End Examinations with 60% marks in the second part. The Course having Practical training will have Practical Examination for 50 marks at the end of Semester, out of which 30 marks for the Practical task assigned at the time of examination. The 20 marks are allotted as Internal Assessment. The allocation of marks for the Internal Assessment and Semester End Examinations are as shown below:Internal Assessment: It is defined as the assessment of the learners on the basis of continuous evaluation as envisaged in the Credit based system by way of participation of learners in various academic and correlated activities in the given semester of the progamme. Semester End Assessment : It is defined as the assessment of the learners on the basis of Performance in the semester end Theory/ written/ Practical examination. Modality of Assessment : Internal Assessment - 40%
Sr No 1 2 3 4
Sr No 1 2 3
40 marks.
a) Theory 40 marks Evaluation type Marks Two Assignments/Case study/Project 20 One class Test (multiple choice questions objective) 10 Active participation in routine class instructional 05 deliveries(case studies/ seminars//presentation) Overall conduct as a responsible student, manners, skill in 05 articulation, leadership qualities demonstrated through organizing co-curricular activities, etc. b) Practicals 20 marks Evaluation type Marks Two best practicals 10 Journal 05 Viva 05
B ) External examination - 60 % Semester End Theory Assessment - 60% 60 marks i. Duration - These examinations shall be of two hours duration. ii. Theory question paper pattern :1. There shall be four questions each of 15 marks. On each unit there will be one question & fourth one will be based on entire syllabus. 2. All questions shall be compulsory with internal choice within the questions. Each question will be of 20 to 23 marks with options. 3. Questions may be sub divided into sub questions a, b, c, d & e only & the allocation of marks depends on the weightage of the topic. Practical External Assessment
30 marks
Note: • A candidate will be allowed to appear for the practical examinations only if he/she submits a certified journal of F.Y.B.Sc. Biotechnology or a certificate from the Head of the department / Institute to the effect that the candidate has completed the practical course of F.Y.B.Sc. Biotechnology as per the minimum requirements. In case of loss of journal a candidate must produce a certificate from the Head of the department /Institute that the practicals for the academic year were completed by the student. However such a candidate will be allowed to appear for the practical examination but the marks allotted for the journal will not be granted.
UNIVERSITY OF MUMBAI
Syllabus for the F.Y.B.Sc. Program: B.Sc. Course : Biotechnology (Credit Based Semester and Grading System with effect from the academic year 2011–2012)
SEMESTER I (Theory) USBT101 PAPER I Unit – I: Microbiology Topic Subtopic A) Nutrition, Nutritional requirements, isolation Nutritional classification of microorganisms. cultivation and Design of culture media, types of culture media. preservation of Concept of isolation, methods of isolation- natural microorganisms microbial populations (mixed culture). Cultivation of microorganisms , Methods of enumeration, Preservation of microorganisms (Principles and method) B) Sterilization i) Principle and application – methods of and disinfection sterilization (physical, chemical, filtration, heat, radiation, gaseous, etc). ii) Disinfection – ideal disinfectant, list of disinfectant, and their application – evaluation of disinfection (Phenol coefficient) Unit – II: Genetics Topic Subtopic A) Mendelian i)Segregation- Mendels experiments, terminology, principles testing phenotypes, examples of gene differences and segregation. ii) Independent assortment – genotypes of dihybrid crosses, testing dihybrid genotypes, crosses involving 3 -4 gene differences, correspondence between mendelian factors and chromosome, symbols, segregation and assortment in haploid organisms. B) Mutations Types of mutations, mutagen, types of mutagens, and mutagenic molecular basis of mutagenesis, reversion, agents induced and spontaneous mutation and silent mutation. Unit – III: Microbial diversity and Functional Biology Topic Subtopic A) Different i) Archaebacteria groups of ii) Eubacteria prokaryotes iii) Blue green algae, Actinomycetes, Eumycota B) Plants Plant cell, types of plant tissues and their importance, organization on angiosperms and modifications of organs of commercial importance Storage roots like sweet potato, carrot, beetroot, raddish. Storage stems – potato, ginger, turmeric, yams, onion, garlic, Acorus, Fruits – Parthenocarpy Seeds – (lectin) red bean
No. of Lectures 45 15
15
15
No. of credit 2
SEMESTER I (Theory) USBT102
Topic A) Ultra structure of prokaryotes B) Ultra structure of Eukaryotic cell Topic A) Chemical bonds and Role of water
PAPER – II Unit – I: Cell Biology Subtopic Flagella, Pilli, capsule, cell wall, cell membrane, outer membrane, cytoplasm, endospores, reserve material, mesosome, nuclear material, plasmid extrachromosomal material Structure of Fungal cell and Yeast – Cell wall, plasma membrane.
Unit – II: Biomolecules Subtopic Covalent bonds, dipoles, ionic, hydrogen bonds. Hydrophobic interaction, Vander Waals forces, functional group. Structure and properties of water, pH – pH meter, Buffers.
No. of Lectures 45 15
15
B) D & L Glyceraldehydes, structure of Carbohydrates monosaccharide, disaccharides, and polysaccharides. Isomers of monosaccharides, chemical/physical properties of carbohydrate, chemical reactions for detection of mono., di and polysaccharides C) Nucleic acids
Structure, function of NA, properties and types of DNA, RNA, structure of polynucleotides
Unit – III: Medical Biotechnology Subtopic A) What is biotechnology? Introduction Biotechnology as an interdisciplinary pursuit. to Scope of biotech in various areas. Biotechnolog Public perception of biotechnology. y and its areas Biotechnology and the developing world. of application B) HostHost parasite relations, infections: sources, Microbe methods of transmission, virulence factors, basic interaction types of clinical infections, Topic
15
No. of credit 2
SEMESTER –I (Practicals) USBTP1
No. of credits
No. 1
2.
3. 4. 5.
6.
PAPER I Title Study of Microscope, dark field microscope and all Lab equipmentsautoclave, hot air oven, centrifuge, incubator, rotary shaker, filter assembly, LAF, pH meter Media preparation and sterilization –Nutrient broth/agar, Sabourauds broth/agar, MacConkeys broth/agar, Superimposed imposed blood agarslant and plate Aseptic transfer of sterile Nutrient Agar/St. Nutrient broth Study of plant tissues from suitable material- parenchyma, sclerenchyma, xylem and phloem. Enrichment (Allen and Chu) of algae and permanent slides of - Nostoc, Anabaena, Spirullina, Chorella
1
Slide culture technique for Actinomycetes
SEMESTER –I (Practicals) USBTP1
No. of credits
3
PAPER II Title Simple staining – Monochrome stain with basic fuchin, crystal violet, malachite green, safranin.-yeast. Differential staining – Grams staining of mixture (gram positive and gram negative) Study of fungi (Aspergillus niger) and yeast.
4 5
Special staining – capsule, cell wall, lipid, spores, nucleus Motility –hanging drop, stab culture.
6
Qualitative tests for biomolecules – carbohydrates, lipids
7 8
Isolation of organism from stool/feces on selective medium (Mac Conkey agar) Gram staining of organism from saliva and skin.
9
Extraction of Pectin from any suitable fruit.
10
Staining of Starch grain from potato
No. 1 2
1
Total number of credits
2.0
SEMISTER II (Theory) USBT201
Topic A) The Microscope
B)Stains and Staining solution
PAPER – I Unit – I: Microbiology Subtopic Lenses and bending of light, Simple and compound microscope, Light Microscope (Bright –field microscope) Parts of light microscope- Eye piece, objectives, condensors, numerical aperture, resolving power, Dark field microscope.
No. of No. of Lectures credit 45 2 15
Definition of dye, chromogen. Structure of dye. Chromophore and auxochrome groups. Preparation and staining of specimens, Fixation, Dyes and simple stains, Leuco compound, Monochrome staining, Mordant, Differential staining (Gram’s and Acid-fast), Romanowsky’s stain, natural dyes. Unit II:Genetics Subtopic Transformation- Griffith’s exp. Conjugation-Davis experiment, Transduction-generalised (basic concept)
15
Unit– III: Microbial diversity and Functional Biology Topic Subtopic A) Histology Animal cell, tissues – epithelial tissues, connective of tissues, muscular tissues, nervous tissues. mammalian tissues B) What organisms are suitable for genetic Experimental experimentation: eukaryotes and prokaryotes. models Criteria for selection Maintenance of Drosophila, Albino mice, Guinea pigs, Hamsters, Monkey, Saccharomyces cerevisiae, neurospora crassa, Zea mays, Pisum sativum, E.coli
15
Topic A) Gene transfer mechanisms in bacteria B) Extension Multiple alleles-blood group, modification of of genetic dominant relationships, gene interactions, essential analysis and lethal genes, gene expression and environment(temperature, light, hormones)
SEMESTER II (Theory) USBT202
Topic A) Cytoplasmi c organelles B) Interface nucleus including (Cell cycle)
Topic A) Lipids
B) Amino acids, proteins and c0 C) Enzymes
Topic A) Immunity
B) Antigens and antibody
PAPER II Unit– I: Cell Biology Subtopic Introduction to cytoplasmic Mitochondria and chloroplast.
No. of Lectures 45 15 organelles-
Nucleosome, biological significance of DNA, cell cycle- phases, regulation by cell growth and extra cellular signals, cell cycle check points, coupling of s phase to m phase, cell cycle progression. Unit – II: Biomolecules Subtopic Classification and properties, saturated, unsaturated, structure and function triacylglycerol, storage lipids, structural lipids, phospholipids, action of phospholipases, steroids.
15
Structure, properties, function, and chemical reaction of amino acids, classification and structure of proteins. Silk fibroin, keratin hemoglobin and myoglobin. Structure of peptides. Titration curve of amino acids. Concept of Isoelectric pH, zwitter ion. Introduction, classification, active site and enzyme specificity Unit – III: Medical Biotechnology Subtopic Introduction, mechanism of innate immunity, acquired immunity, local and herd immunity. Humoral and cellular immunity. Determinant of antigenicity, biological classes Structure, immunoglobulin classes.
15
No. of credit 2
SEMESTER II (Practicals) USBTP2
No. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
PAPER I Title Study of minimum nutritional requirement. Isolation techniques of E.coli/ S. aureus Preservation of microorganisms – serial subculture method and paraffin oil technique. Serial dilution technique – i) Surface spread ii) pour plate. Enumeration methods – haemocytometer, breeds count, opacity tube Effects of environment on bacterial growth – effect of pH, temp, osmotic pressure Study of aerobic microflora and anaerobic microflora from (cow dung) in Robertson’s cooked meat medium Drosophila culture-corn meal medium Mounting of Squamous and stratified epithelium Study of animal tissues from permanent slides:-blood, bone marrow, neuron, connective tissue-spongy, smooth, skeletal and heart muscles. SEMESTER II (Practicals) USBTP2
No. 1 2 3 4 5
No. of credit 1
PAPER II Title Qualitative tests for biomolecules – protein, nucleic acids, amino acid Amylase, urease, invertase, catalase, dihydrogenase activity (qualitative) Extraction of Casein from milk. Stages of Mitosis Meat tenderization using papain. Total number of credits
No. of credit 1
2.0
Distribution of Topics and Credits F.Y.B.Sc. Biotechnology Semester I Course USBT1O1
Nomenclature Microbiology, Genetics and Microbial Diversity and Functional Biology
USBT1O2
Cell Biology, Biomolecules and Medical Biotechnology
USBTP1
Microbiology, Genetics and Microbial Diversity and Functional Biology Cell Biology, Biomolecules and Medical Biotechnology Practical I & II
Credits 02
Topics 1. Microbiology 2. Genetics 3. Microbial Diversity and Functional Biology
02
1. Cell Biology 2. Biomolecules 3. Medical Biotechnology
02
F.Y.B.Sc. Biotechnology Semester II Course USBT2O1
USBT2O2
USBTP2
Nomenclature Microbiology, Genetics and Microbial Diversity and Functional Biology
Cell Biology, Biomolecules and Medical Biotechnology Microbiology, Genetics and Microbial Diversity and Functional Biology Cell Biology, Biomolecules and Medical Biotechnology Practical I & II
Credits
02
02
02
Topics 1. Microbiology 2. Genetics 3. Microbial Diversity and Functional Biology 1. Cell Biology 2. Biomolecules 3. Medical Biotechnology
Scheme of Examination: The performance of the learners shall be evaluated into two parts. The learner’s performance shall be assessed by Internal Assessment with 40% marks in the first part & by conducting the Semester End Examinations with 60% marks in the second part. The Course having Practical training will have Practical Examination for 50 marks at the end of Semester, out of which 30 marks for the Practical task assigned at the time of examination. The 20 marks are allotted as Internal Assessment. The allocation of marks for the Internal Assessment and Semester End Examinations are as shown below:Internal Assessment: It is defined as the assessment of the learners on the basis of continuous evaluation as envisaged in the Credit based system by way of participation of learners in various academic and correlated activities in the given semester of the progamme. Semester End Assessment : It is defined as the assessment of the learners on the basis of Performance in the semester end Theory/ written/ Practical examination. Modality of Assessment : Internal Assessment - 40%
Sr No 1 2 3 4
Sr No 1 2 3
40 marks.
a) Theory 40 marks Evaluation type Marks Two Assignments/Case study/Project 20 One class Test (multiple choice questions objective) 10 Active participation in routine class instructional 05 deliveries(case studies/ seminars//presentation) Overall conduct as a responsible student, manners, skill in 05 articulation, leadership qualities demonstrated through organizing co-curricular activities, etc. b) Practicals 20 marks Evaluation type Marks Two best practicals 10 Journal 05 Viva 05
B ) External examination - 60 % Semester End Theory Assessment - 60% 60 marks i. Duration - These examinations shall be of two hours duration. ii. Theory question paper pattern :1. There shall be four questions each of 15 marks. On each unit there will be one question & fourth one will be based on entire syllabus. 2. All questions shall be compulsory with internal choice within the questions. Each question will be of 20 to 23 marks with options. 3. Questions may be sub divided into sub questions a, b, c, d & e only & the allocation of marks depends on the weightage of the topic. Practical External Assessment
30 marks
Note: • A candidate will be allowed to appear for the practical examinations only if he/she submits a certified journal of F.Y.B.Sc. Biotechnology or a certificate from the Head of the department / Institute to the effect that the candidate has completed the practical course of F.Y.B.Sc. Biotechnology as per the minimum requirements. In case of loss of journal a candidate must produce a certificate from the Head of the department /Institute that the practicals for the academic year were completed by the student. However such a candidate will be allowed to appear for the practical examination but the marks allotted for the journal will not be granted.
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